Chromatography {liquid-liquid chromatography} {partition chromatography} can be for polar molecules. It has high resolution, uses small batches, is more reproducible, and uses lower concentration than adsorption chromatography. Stationary phase is deactivated silica gel, diatomaceous earth, or cellulose, which absorb water. Organic solvents, such as octanol, slightly soluble in water are mobile phase. Solute has relative concentrations in octanol and water. Polarity, hydrogen bonding, and molecule size affect partition. Concentration ratio {partition coefficient} determines mobility.
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Date Modified: 2022.0224