Liquid-liquid chromatography {high-pressure liquid chromatography} (HPLC) can use high pressure. The 100-atmosphere pressure requires special packing materials, such as Zipax or Coracil bonded beads, with porous-material coatings. Beads can have different sizes. Solvent, pH, ionic strength, and temperature affect HPLC.
purposes
HPLC separates molecules with low vapor pressure and high molecular mass or easily decomposed materials, such as nucleic acids, amino acids, bile acids, drugs, pesticides, herbicides, surfactants, and anti-oxidants. It is fast, is sensitive, and has high resolution.
Physical Sciences>Chemistry>Analytical Chemistry>Chromatography>Kinds
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Date Modified: 2022.0224