300-base to 500-base sequences {expressed sequence tag} (EST) are specific to expressed gene regions, typically at 3' ends. ESTs map gene chromosomal locations from several tissues, recover corresponding gene sequences by electronic-database similarity searching, and retrieve complete cDNA clones for further analysis. Whole-genome shotgun sequencing using EST assembly reduces redundancy and creates longer consensus sequences.
Biological Sciences>Genetics>Recombinant DNA>Gene Expression
4-Genetics-Recombinant DNA-Gene Expression
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Date Modified: 2022.0224