Two complementary DNA or RNA single strands can form a double-stranded molecule {hybridization, DNA}|. Two nucleic-acid strands can pair by hydrogen-bonding A and T, or A and U in RNA, and C and G. DNA or DNA and RNA complementary-strand nitrogenous bases can have hydrogen bonding.
arrays
All array spots have hybridization, as cartridge holds array in position. Hybridization measurement depends on dye sensitivity and dynamic range. If array spots are close together, they can cross-hybridize. Evaporation also causes problems, so arrays have humectants, lids, or dewpoint controls. Spotting pins must be 0.2 mm small and clean. Alternatives to spotting include acoustic focusing, multi-nozzle piezoelectric jets, and continuous solid pin spotting. Probes, cDNA arrays, and oligonucleotide arrays are alternative hybridization methods.
Biological Sciences>Genetics>Recombinant DNA>Amplification
4-Genetics-Recombinant DNA-Amplification
Outline of Knowledge Database Home Page
Description of Outline of Knowledge Database
Date Modified: 2022.0224