Techniques {amplification, DNA} {DNA amplification} can increase number of DNA-fragment copies. In vivo amplification amplifies repeat sequences in fragile X syndrome. In vitro amplification uses cloning or polymerase chain reactions.
purposes
Amplified DNA indicates mutations, translocations, viral and bacterial infections, sex, genealogy, living and extinct species differences, and forensic identification.
DNA synthesis
To make probes or primers, first blocking-group covers 5' or 3' hydroxyl groups of two nucleotides. Then other two hydroxyl-groups react to make phosphodiester bonds. Then acid or base removes blockers. Process repeats to elongate chain.
Biological Sciences>Genetics>Recombinant DNA>Amplification
4-Genetics-Recombinant DNA-Amplification
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Date Modified: 2022.0224